Journal article
Utility of the Sindbis replicon system as an Env-targeted HIV vaccine
RJ Center, A Miller, AK Wheatley, SM Campbell, C Siebentritt, DFJ Purcell
Vaccine | ELSEVIER SCI LTD | Published : 2013
Abstract
Sindbis replicon-based vaccine vectors are designed to combine the immunostimulatory properties of replicating viruses with the superior safety profile of non-replicating systems. In this study we performed a detailed assessment of Sindbis (SIN) replicon vectors expressing HIV-1 envelope protein (Env) for the induction of cell-mediated and humoral immune responses in a small animal model. SIN-derived virus-like particles (VLP) elicited Env-specific antibody responses that were detectable after boosting with recombinant Env protein. This priming effect could be mediated by replicon activity alone but may be enhanced by Env attached to the surface of VLP, offering a potential advantage for thi..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
We thank Aalto Bio Reagents for the gift of FITC-labeled monoclonal anti-gp120, Dr Carol Hartley (University of Melbourne, Australia) for providing non-immune sheep sera, and Sondra Schlesinger (Washington University, St. Louis, USA) for providing the pSINrep5, pDHBB and pDH26S plasmids and Prof. Stephen Kent (University of Melbourne, Australia) for critical review of the manuscript. HIV-Ig was obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH from NABI and NHLBI (Catalog #3957). This work was supported by the National Health and Medical Research Council of Australia through Program Grant #510448.